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Cellular stress and AMPK activation as a common mechanism of action linking the effects of metformin and diverse compounds that alleviate accelerated aging defects in Hutchinson-Gilford progeria syndrome

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Both experimental and clinical studies have reported iron accumulation, enhanced lipid peroxidation, and reduced glutathione peroxidase 4 ( GPX4 ) expression in DKD kidney tissue, supporting ferroptosis as a potential mechanistic link between tubular injury, oxidative stress, and renal fibrosis (Wu and Chen, 2022)

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For cells treatments, AOAA (Selleck Chemicals LLC, Houston TX 77230 USA), I3MT-3 (MedChemExpress, Sollentuna, Sweden ) , and Erastin ( Tocris-Bio-Techne SRL, Milano, Italy) were solubilized in DMSO