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A 2.22 RT buffer was freshly prepared, consisting of 2.22 MaP pre-buffer (5 MaP pre-buffer: 250 mM Tris-HCl pH 8.0, 375 mM KCl, 50 mM DTT), 2.22 M betaine, and 11.1 mM MgCl

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Your provider determines which is a better fit based on your history and goals

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