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We transduced primary hepatocytes with a recombinant adenovirus expressing NHK under doxycycline (Dox)-inducible control (Ad-TetOn-NHK)

So far, the results are looking really, really good

Cheeks flatten, temples hollow and tear troughs deepen

The R groups that can be protonated/deprotonated include Lys, Arg, and His, which have a + 1 charge when protonated, and Glu and Asp (carboxylic acids), Tyr and Ser (alcohols), and Cys (thiol), which have zero charges when protonated

It provides the bioavailability needed for these effects

After alcohol administration, 10 responses on the alcohol-appropriate lever (e.g., correct lever) resulted in sucrose delivery, while responses on the other (e.g., incorrect lever) produced no programmed consequence