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Nucleofections were performed with a protocol optimized for MEFs using the Neon electroporation system (Invitrogen) (cells resuspended in Gibco 1xPBS, 1350 mV, 1 pulse, 30 ms) or Amaxa nucleofection system (Lonza) with proprietary nucleofection protocols optimized for HCT116 or MEFs, and cells resuspended in Solution I (5 mM KCl, 15 mM MgCl 2 , 50 mM Mannitol, 120 mM sodium phosphate solution pH 7.2)

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Research indicates that GHK-Cu influences angiogenesis in the nervous system, nerve outgrowth, and central nervous system inflammation

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significant elevation in circulating glutathione levels can be achieved at a significantly lower cost than any of the existing examples and overcome the regulatory obstacle of structurally modifying glutathione to increase or enhance its bioavailability

It's supposedly the homocysteine coming out of the system