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injection of 50 mg/kg sodium pentobarbital (Sigma), and thoracic aorta was carefully dissected and maintained at 37 C in Krebs solution containing 118.2 mM NaCl, 4.7 mM KCl, 1.2 mM MgSO 4 , 25 mM NaHCO 3 , 1.2 mM KH 2 PO 4 , 1.9 mM CaCl 2 and 11.7 mM dextrose (pH 7.4, 95% O 2 , 5% CO 2 )

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Consider: Maintaining a consistent injection day and time each week Discussing with your GP whether adjusting injection timing might help Dietary modifications can significantly reduce gastrointestinal side effects that interfere with sleep: Eat smaller, more frequent meals throughout the day Avoid large meals within 34 hours of bedtime Limit fatty, spicy, or rich foods, particularly in the evening Stay well hydrated during the day but reduce fluid intake before bed Avoid caffeine and alcohol, which can exacerbate both gastrointestinal symptoms and sleep problems Blood glucose monitoring is essential, particularly if you experience symptoms suggestive of nocturnal hypoglycaemia

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