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After 24 h of incubation, cells were labeled with 10 M C11BODIPY at 37 C for 30 min in darkness, subsequently rinsed with prechilled PBS, harvested, and analyzed for fluorescence intensity via CytoFLEX flow cytometry (Beckman Coulter)
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We subsequently performed quantitative measurement of ONSD using standardized amplitude modulation (A-scan) echography with tissue sensitivity settings, placing the 8 MHz A-scan probe on the temporal eye equator in primary gaze position, as previously described by others [24, 25]
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He or she will be able to help you weigh the benefits against the risks in your specific case
Recent research published in BMJ Medicine suggests that GLP-1 medications may support heart health, with cardiovascular benefits increasing with longer duration of use
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