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In: Shils ME, Shike M, Ross AC, Caballero B, Cousins RJ, eds

Mobile phase A: ammonium acetate 100 mM and mobile phase B: ACN/ammonium acetate 100 mM (95/5, v/v) with a 1 ml/min flow and a gradient of 080% in 10 min (Figure 6)

Dosing information Usual Adult Dose for Pernicious Anemia: Initial dose: 100 mcg intramuscularly or deep subcutaneous once a day for 6 to 7 days If clinical improvement and reticulocyte response is seen from the above dosing: 100 mcg every other day for 7 doses, then: 100 mcg every 3 to 4 days for 2 to 3 weeks, then: Maintenance dose: 100 to 1000 mcg monthly Duration of therapy: Life Comments: Administer concomitant folic acid if needed

No standardised human research protocol exists

When these gastrointestinal side effects happen, they are typically mild in nature and generally subside over time, usually within 2-3 weeks