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As a result, methylation may be disrupted

At pH 9, CVA21 was eluted at 300 mM NaCl, while at pH 7, CVA21 was partially eluted at 1,000 mM NaCl and in the subsequent 1 mM GSH elution buffer

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The appearance of lyophilized powder can vary from a solid, dense puck to a more diffuse powder, but this does not affect the mass

The collected diffraction patterns were subjected to background correction and standard smoothing, and the resulting data were analyzed using DIFFRAC.EVA V4.1 software, comparing them to both the PDF-2 commercial database and an in-house reference library