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Cells were then incubated overnight at 4C with different pairs of the following antibodies: mouse anti H2AX (Millipore cat.# 05-636), rabbit anti ICP8 antibody (kindly provided by prof WT Ruyechan, Univesity of Buffalo, Buffalo, NY, USA), as primary antibodies and Cy2-conjugated donkey anti-rabbit IgG and Alexa Fluor 568 donkey anti-mouse IgG (Molecular Probes, Life Technologies), as secondary antibodies (30 min at room temperature)
D., Colwell, Iqbal, Z
Rush-Monroe K
pylori were grown for 16 h at 37C and diluted to OD 600 = 0.1 in Brucella broth containing 10% (v/v) FBS HI and 50 g/mL glutathione (glycine- 13 C 2 , 15 N) (95%+ purity, Cambridge Isotope Laboratories
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