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If the protocol calls for one dose per day, administer it at the same time each day

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Then, membranes were incubated with appropriate secondary antibodies, HRP-goat-anti-mouse (Abcam, ab6789) or HRP-goat-anti-rabbit (Abcam, ab6721), to bind the primary antibodies for 1 h at room temperature

He identified tarsal tunnel syndrome that had been missed for years