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Subsequently, qRT-PCR was performed with SYBR Green Master Mix (RR820A, Takara), wherein GLP-1 and MPC2 expression levels were normalized to GAPDH and calculated via the 2 CT method

Curr Opin Neurol

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This process occurs independent of overall weight loss, meaning liver fat reduction begins before significant scale changes become apparent

Understanding the unique biology of these subtypes is critical for developing targeted therapies and improving patient outcomes