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Immunostaining was performed by seeding cells on glass coverslips, fixing with paraformaldehyde, permeabilizing with Triton X-100, and then incubating with primary anti-NF-B p65 antibody, followed by Alexa Fluor 546-conjugated secondary antibody

Sleep quality, stress levels and recovery habits strongly influence inflammation and tissue repair

Freezing damages the peptide structure and reduces bioactivity

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There is no universally agreed-upon clinical protocol for human use, as epithalon has not undergone large-scale phase III clinical trials in Western countries