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Usually, it's suggested to detect prepared sample immediately or aliquot sample to refrigerate in a disposable container

Mind the Shelf Life: While bacteriostatic water prevents bacterial growth, the peptide molecule itself will still degrade over time

Without bacteriostatic water, the solution becomes contaminated within 2448 hours even under refrigeration, creating infection risk at the injection site

Peroxynitrite (ONOO - ,0.1 M stock solution in 0.3 M NaOH-NaCl) was diluted to less than 1/100 with ice-cold water, just prior to quick exposure to HGF in PBS by vortexing at 1:2000 (see Figure 1a for the experimental design) 32

The number of viable cells was measured by flow cytometry after addition of 5 l spike in fluorescently labeled bead (CountBright Plus Absolute Counting Beads, ThermoFisher Scientific) for normalization

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