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Beads were repeatedly washed with GST-lysis buffer, prepared for SDS-PAGE, and finally analyzed by Western blot using antibodies against GFP, mRFP, and Flag-tag when needed, as well as HRP-conjugated secondary antibodies for ECL detection

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You may retain water for a number of reasons, including: standing or sitting in the same position for too long eating too much salty food having overweight being pregnant taking certain medications, such as: some blood pressure medications contraceptive pills hormone therapy antidepressants steroids Over time, weight loss may cause your metabolism to slow, significantly decreasing the rate at which you lose weight

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