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Then, tissues were homogenized using a pestle, and the homogenate was maintained on ice throughout the procedure to prevent protease activity
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24 This study has been criticized because a high dropout rate biases findings in a positive direction
Thermal equilibration was then conducted for 20 ps, gradually increasing the temperature from 100 K to 298 K using the Langevin thermostat (collision frequency of 1 ps 1 )
In atopic asthma, allergens release proteases, which disrupt the epithelial barrier and induce secretion of alarmins such as interleukin-25 (IL-25), interleukin-33 (IL-33), and thymic stromal lymphopoietin (TSLP) from epithelial cells
