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This significant growth reflects increasing adoption of Tirzepatide for managing type 2 diabetes and obesity, driven by its efficacy and favorable patient outcomes

Probe 1426 in the presence of Ampligase (Lucigen) was used to selectively circularize and protect fragments containing the barcoded transposon sequence, while all other fragments were digested with a mix of T7 gene 6 exonuclease (ThermoFisher), Lambda exonuclease (NEB), and Exonuclease I (NEB)

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However, the mechanism of activation still remains unclear

doi: 10.1034/j.1399-3054.2002.1140201.x 8 Clemente-MorenoM