The efficacy and results will depend upon consistent use
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This dehydrogenase reaction occurs in the cytoplasm and requires NAD+ as a cofactor, which is reduced to NADH in the process
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The container must be rigid, leak-resistant, puncture-resistant, with a tight screw cap, and clearly labeled (e.g., SHARPS DO NOT RECYCLE)
In the BBB model with iNHA, the bottom of the inserts was precoated with 0.001% Poly-L-Lysine (L 7240, Biochrom AG, Darmstadt, Germany) for 45 min at room temperature