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The trials tested oral, intravenous, and subcutaneous routes of administration at doses ranging from micrograms to 1 mg per day

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The culture device was placed in a custom-made incubator that maintains a temperature of 37 C, and tissue was maintained half-soaked in sterile, serum-free culture medium (Iscoves Modified Dulbeccos Medium without phenol-red (IMDM, GIBCO) supplemented with 20% KnockOut serum replacement (GIBCO), 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine, 1% non-essential amino acids, 1% HEPES) using a syringe pump

IL-17-mediated signaling in astrocytes promotes the secretion of MMP-3 and MMP-9, further compromising BBB integrity