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In step-e) cleavage is carried out for protected peptide from solid support resin using a reagent to obtain Fragment -7

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Chromatographic separation was archived on an ACQUITY UHPLC HSS T3 column (100mm2.1 mm, 1.8um, Waters) with column temperature kept at 40 and injection volume of 5uL

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3 However, their reduced appetites dont last after they stop taking the medication