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Plasma enzyme assay and liver histology Plasma was extracted from whole blood and immediately measured for alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities using a biochemical kit (Diagnostic Chemicals Ltd., Oxford, CT)

Use immediately after reconstitution or aliquot and store at -20C

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Next, to further investigate the role of Trim37 in PGCs in vivo, we first generated Blimp1-T2A-Cre knock-in mice and validated Cre-mediated loxP recombination in gonads using Rainbow mice 29 (Supplementary information, Fig