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Following signal amplification, opal dyes [Opal 520 (OP-001001), Opal 570 (OP-001003), and Opal 690 (OP-001006) (Akoya, Marlborough, MA)] were diluted in TSA buffer (1:1000) and applied to the sections

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In one study rats were exposed to 2% solutions of -PGN, -PGN and -PGN in their drinking water for 2 to 4 weeks

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They represent a local concentration of the peptide solution in the subcutaneous layer rather than rapid dispersion