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Step 3: Allow several minutes the serum to absorb

Davis and Parish, 2013

Then, the cells were harvested and lysed with 0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5 to afford a protein free lysate

Inflammation and injury to the joint cause changes in bone-joint structure, deterioration of tendons and ligaments, and a breakdown of cartilage