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Nature 388 , 882887 (1997)

PMID: 29998800 Seiwerth S, Brcic L, Batelja Vuletic L, et al

Vitamin B12 is an essential nutrient that plays a critical role in the bodys energy production

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PMC 434288

The chip preparation procedure included: Conditioning: 50 mM NaOH Activation: EDC/NHS solution Capture: Strep-Tactin XT (50 g/mL in 10 mM sodium acetate, pH 4.5) Quenching: 1 M ethanolamine hydrochloride, pH 8.5 Wash: 0.1 M sodium borate, 1 M NaCl, pH 9.0 Twin-Strep-tagged ligands were captured on the chip using a 96-channel printhead under bidirectional flow for 750 s, followed by a 600 s baseline step in running buffer