Clin Cancer Res 26:274281 Kanamori M, Higa T, Sonoda Y, Murakami S, Dodo M, Kitamura H, Taguchi K, Shibata T, Watanabe M, Suzuki H, Shibahara I, Saito R, Yamashita Y, Kumabe T, Yamamoto M, Motohashi H, Tominaga T (2015) Activation of the NRF2 pathway and its impact on the prognosis of anaplastic glioma patients
B Biomed
If you think you will be purifying the same exact protein again in the future, then you should rinse your elution buffer out with a few column volumes of deionized water (dIH 2 O), and then store the beads in 20% ethanol until youre ready to use them again
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Taking the vial from a cold fridge or freezer and immediately injecting room-temperature liquid can cause condensation to form
Get the reconstitution wrong and you are guessing at every dose