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The tools we use to measure lean mass in clinical trials (DXA) likely overestimate the muscle component of LBM loss because they cannot distinguish liver and muscle substrate changes from actual contractile protein loss

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Extraction was performed by rotation of tubes at room temperature by 24 h, at 30 rpm in Multi RS-60 Rotator (Biosan, Riga, Latvia) and next centrifugation at relative centrifugal force 12,333g by 10 min using an Eppendorf 5810R centrifuge (Eppendorf, Hamburg, Germany)

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Each month Ive increased the dosage, and once I started 1.0 mg my [nausea] was at its height, Lynnette says