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The GSH-Glo Glutathione Assay GSH Depletion and Recovery in Treated Cells HeLa cells (5000 cells/well in 96 well format) were exposed to l-Buthionine-sulfoximine (BSO)

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To elucidate the relevance of charge and size of the side chain of the scaffold candidate, we replaced Glu170 of ScGrx7 with Asp, Ala or Lys