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using the peptide beyond 28 days introduces variables related to oxidation that can confound sensitive metabolic data

Insulin secretion in response to treatment with 10 nM GLP-1 analogs was determined, after another 2-hour incubation of cells in KRB buffer containing low and high glucose, using an enzyme-linked immunosorbent assay kit according to the manufacturer's instructions (Millipore, Billerica, MA, USA)

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If you attempt to save half for later, bacteria can multiply rapidly in the remaining liquid

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