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Companion satellite-cell cultures, prepared at the same time, were immunostained at 30-h post-plating with D3 anti-desmin mAb and DAB (a chromogenic HRP-substrate that forms an insoluble brown reaction product) to determine the percentage of myogenic cells present 18 ,19 cultures with less than 95% DAB-positive cells (namely, less than 95% desmin-positive myogenic purity) were not used for experiments just to ensure the reliability of data specific to myogenic cells in the employed primary cell culture systems

Yi xue ban = Journal of Zhejiang University

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doi: 10.1212/01.wnl.0000252807.38124.a3 4

Consult your healthcare provider before making any medication changes

It is because your body has lost its adaptation to the GI-slowing effects, and jumping back to a full dose will likely cause the same nausea you experienced at the start