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coli -treated, Streptococcus anginosus ( S

10.1007/s00011-018-1146-7 62 DinnJ

Such elevation in intracellular calcium and the probable activation of PKC are suggested to culminate in the observed exocytosis of vesicles containing growth hormone from pituitary cells

HEK293T cells were transfected with 1 g lentiCRISPRv2 containing the sgRNA insert, 0.67 g psPAX2 (Addgene, 12260) and 0.33 g pVSVg (Addgene, 8454) using 6 g polyethylenimine (Sigma-Aldrich, 919012) in Opti-MEM (Thermo Fisher, 31985062)

Ten micrograms of each protein sample were separated into a 10% SDS-PAGE gel and transferred onto a polyvinylidene fluoride membrane