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Effect of renal impairment on the pharmacokinetics of a single oral dose of danuglipron in participants with type 2 diabetes

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Western immunoblot analyses For the preparation of total liver homogenates, frozen liver pieces were homogenized in RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 1 mM EDTA, 1 mM PMSF, protease inhibitor cocktail, pH 7.4) on ice with a tissue tearor (BioSpec Products, Bartlesville)

Gastroenterology Research and Practice, 2013

In comparison to control diet, HFD consumption resulted in significant reductions in both NF-B-DNA-binding activity (Fig