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The assay uses an enzymatic method with Ellman's Reagent (DTNB) and glutathione reductase (GR): DTNB reacts with reduced glutathione to form a yellow product, and the rate of change in optical density at 412 nm is directly proportional to the glutathione concentration

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The GLP-1 hormone plays a key role in blood sugar regulation by stimulating insulin production in response to food intake, slowing down digestion, and reducing the release of glucagon, a hormone that raises blood sugar levels

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