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Multiple and additive functions of ALDH3A1 and ALDH1A1: cataract phenotype and ocular oxidative damage in Aldh3a1(/)/Aldh1a1(/) knock-out mice

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Other GLP-1 benefits besides weight loss Written by: Morgan Pennington , 10 Mar 2025 5 min read Reviewed and fact-checked: Niya Mansuri , Prescribing Pharmacist, GPhC No

Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark