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Master mix and Taqman gene expression assays for glyceraldehyde 3-phosphate dehydrogenase (GAPDH control) and target genes occludin (Hs05465837_g1) and claudin-1(Hs00221623_m1) were obtained from Applied Biosystems (Foster City, CA, USA)
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& Gorman, H
Secretions are scored from 0 to 2, with 0 for no secretion, 1 for serous discharge, and 2 for purulent discharge
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Research endpoints include epithelial-barrier integrity, cytoprotective signaling, inflammatory-response markers, peptide biodistribution, and comparisons with parenteral delivery models
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