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doi: 10.2174/092986709788803060
The evidence summary below is per-compound only, drawn from the Phase 3 retatrutide program and the preclinical-plus-analog MOTS-c literature
Cells on poly-D-lysine-coated coverslips were loaded with 2 M Fura-2 AM (Thermo Fisher Scientific, Waltham, MA) at 37 C for 40 min in DMEM
Confocal images were for above described applications were acquired with a DMI6000 microscope (Leica Biosystems, Wetzlar, Germany) and analyzed using ImageJ default plugins for co-localization and staining intensity
Can be used AM and PM
This paradoxical reaction is not fully understood but is thought to be related to individual metabolic responses
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