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A simple system is dividing the abdomen into four quadrants and rotating clockwise each week: right upper, left upper, left lower, right lower

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Cultures were maintained in a humidified atmosphere of 5% CO 2 at 37C for 24 h and then incubated for the next 24-h period in DMEM-10% HS additionally containing recombinant HGF that was treated with peroxynitrite with or without trisulfide pre-treatment (see Figure 1d,f)

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