Funding This work was funded by the Department of Biotechnology (DBT)- BT/PR50342/CMD/150/135/2023
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This creates an extraordinarily high binding affinity with pK = 16.44, approximately 10 million times higher than other tripeptides and comparable to albumins copper binding (pK = 16.2)
The resulting single-stranded cDNAs were used as templates in PCR reactions using gene-specific primers to amplify TGR (forward 5-CTATTTCCGTAGACGTCTGT-3 and reverse 5-AATACAGTTTCCTTCCCGTT-3) or GAPDH (forward 5-GTTTTGGTCGTATCGGGAGA-3 and reverse 5-ATGCGTTAGAAACCACGGAC-3)
Kuchakulla M, Masterson T, Arora H, Kulandavelu S, Ramasamy R