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Carnitine and its derivatives were immediately determined in the supernatant using high-performance liquid chromatography (HPLC, VWR Hitachi, Radnor, Pennsylvania, USA) coupled with mass spectrometry (3200 Q Trap, AB Sciex, Framingham, Massachusetts, USA)

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Many studies have shown that a high-protein diet (typical of Western-style diets) can lead to progressive kidney damage and albumin loss in individuals with suboptimal kidney function