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Since we intended to trap proteins that were oxidized (before reversal) after a very short time, a bolus of high SFN (mM) or H 2 O 2 (mM) was chosen to increase the likelihood of oxidized protein detection (34, 35)
Bioresour Technol 100:350355
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CDC (2023) General Best Practice Guidelines for Immunization: Vaccine Administration Related research, protocols, and guides Explore the available research context, protocol variants or comparisons, and practical guides
Liver samples were then mixed with 4X Laemmli sample buffer (8% SDS, 40% glycerol, 0.4% bromophenol blue, 240 mM Tris, pH 6.8) with freshly added -mercaptoethanol and were heated for 5 mins at 95 C
Il est prsent dans toutes les cellules de lorganisme, o il est essentiel au systme de dfense cellulaire et participe de nombreux processus biologiques
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