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All factors are assessed by a trial clinician before and during participation
10.3389/fimmu.2020.01523 24
For experiments involving caspase-3/7 staining, CellEvent Caspase-3/7 Green Detection Reagent (Thermo Scientific C10723) and CPP-CAND were added to live cells 24 h before imaging using an Eclipse Ti-U inverted microscope (NIKON)
In reproduction, we often focus on the HPO axis the hypothalamus, pituitary gland, and ovaries
Analyses were blinded to treatment conditions
A mechanism in a cell culture or a rat tendon injection is not proof of the same effect in an oral human dose
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