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For those diagnosed with a true deficiency , injections might be needed weekly or monthly until levels are normalised, GP surgeries would typically schedule B12 injectsions every 12 weeks thereafter

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Starting with lower doses and gradually increasing allows the body to adjust and reduces the likelihood of systemic reactions

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RNA was precipitated by incubating the samples with 500 L of ice-cold isopropyl alcohol (Roth) and 100 L cold sodium acetate (3 M, pH 5.5) for 1 h at 20 C

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