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The introduced Nde I and Eco RI restriction sites were used to clone the gene into the pPT-plasmid resulting in pPT_ roGFP2 coding for a TorA_roGFP2 fusion protein
Subsequently, the membranes were washed with TBST and incubated with appropriate horseradish peroxidase-linked secondary antibodies for 2 h at room temperature followed by further washing with TBST
Our Experience: We've seen some patients respond favorably over time to these skin infusions
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